Plasmid_Backbone

Part:BBa_K547000:Design

Designed by: Laurynas VANAGAS   Group: iGEM11_HokkaidoU_Japan   (2011-10-02)


ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2319
    Illegal suffix found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2319
    Illegal SpeI site found at 138
    Illegal PstI site found at 152
    Illegal NotI site found at 145
    Illegal NotI site found at 2325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2319
    Illegal XhoI site found at 1169
    Illegal XhoI site found at 2195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2319
    Illegal suffix found at 138
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2319
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2334
    Illegal SpeI site found at 138
    Illegal PstI site found at 152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2991
    Illegal BsaI.rc site found at 2
    Illegal BsaI.rc site found at 3653


Design Notes

Removed Spe1 restriction site from GSK tag by silent mutation.


Source

SlrP: S. enterica Typhimurium GSK: Glycogen Synthase Kinase 3beta

Julie Torruellas Garcia, Franco Ferracci, Michael W. Jackson,1 Sabrina S. Joseph, Isabelle Pattis, Lisa R. W. Plano, Wolfgang Fischer, and Gregory V. Plano. 2006. Measurement of Effector Protein Injection by Type III and Type IV Secretion Systems by Using a 13-Residue Phosphorylatable Glycogen Synthase Kinase Tag. Infect Immun.Vol.74:5645-57.

References